goat anti human alk1 antibody Search Results


human alk-1 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation human alk-1 antibody
Human Alk 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
human alk-1 antibody - by Bioz Stars, 2026-02
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anti-human alk1  (R&D Systems)
90
R&D Systems anti-human alk1
Anti Human Alk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mouse monoclonal anti-human cd246 alk1  (Agilent technologies)
90
Agilent technologies mouse monoclonal anti-human cd246 alk1
Distribution of immunohistochemical scores with the 3 different clones <t>(ALK1,</t> 5A4 and D5F3).
Mouse Monoclonal Anti Human Cd246 Alk1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-human cd246 alk1/product/Agilent technologies
Average 90 stars, based on 1 article reviews
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alk1 rabbit polyclonal anti-human  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology alk1 rabbit polyclonal anti-human
Distribution of immunohistochemical scores with the 3 different clones <t>(ALK1,</t> 5A4 and D5F3).
Alk1 Rabbit Polyclonal Anti Human, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alk1 rabbit polyclonal anti-human/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
alk1 rabbit polyclonal anti-human - by Bioz Stars, 2026-02
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alk1  (OriGene)
92
OriGene alk1
Distribution of immunohistochemical scores with the 3 different clones <t>(ALK1,</t> 5A4 and D5F3).
Alk1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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source alk1 rabbit  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc source alk1 rabbit
Distribution of immunohistochemical scores with the 3 different clones <t>(ALK1,</t> 5A4 and D5F3).
Source Alk1 Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/source alk1 rabbit/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
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fully human antibody against the extracellular domain of alk1 pf-03446962  (Pfizer Inc)
90
Pfizer Inc fully human antibody against the extracellular domain of alk1 pf-03446962
Distribution of immunohistochemical scores with the 3 different clones <t>(ALK1,</t> 5A4 and D5F3).
Fully Human Antibody Against The Extracellular Domain Of Alk1 Pf 03446962, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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human alk1 inhibitory antibody  (R&D Systems)
92
R&D Systems human alk1 inhibitory antibody
Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the <t>ALK1/2/3/6</t> inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 <t>inhibitory</t> antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.
Human Alk1 Inhibitory Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human alk1 inhibitory antibody/product/R&D Systems
Average 92 stars, based on 1 article reviews
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mouse monoclonal anti-human cd246  (Agilent technologies)
90
Agilent technologies mouse monoclonal anti-human cd246
Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the <t>ALK1/2/3/6</t> inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 <t>inhibitory</t> antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.
Mouse Monoclonal Anti Human Cd246, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-human cd246/product/Agilent technologies
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-human cd246 - by Bioz Stars, 2026-02
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phosphorylated human ampkα2 β2 γ1  (Carna Inc)
96
Carna Inc phosphorylated human ampkα2 β2 γ1
Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the <t>ALK1/2/3/6</t> inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 <t>inhibitory</t> antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.
Phosphorylated Human Ampkα2 β2 γ1, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated human ampkα2 β2 γ1/product/Carna Inc
Average 96 stars, based on 1 article reviews
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alk 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology alk 1
Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the <t>ALK1/2/3/6</t> inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 <t>inhibitory</t> antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.
Alk 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alk 1/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
alk 1 - by Bioz Stars, 2026-02
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flex mouse monoclonal anti-human cd246 alk antibody alk1  (Agilent technologies)
90
Agilent technologies flex mouse monoclonal anti-human cd246 alk antibody alk1
Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the <t>ALK1/2/3/6</t> inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 <t>inhibitory</t> antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.
Flex Mouse Monoclonal Anti Human Cd246 Alk Antibody Alk1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Distribution of immunohistochemical scores with the 3 different clones (ALK1, 5A4 and D5F3).

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Distribution of immunohistochemical scores with the 3 different clones (ALK1, 5A4 and D5F3).

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques: Immunohistochemical staining, Clone Assay

Detailed distribution of immunohistochemical staining score along the entire series of 37 ALK FISH-positive cases according to the 3 different clones (ALK1, 5A4, D5F3).

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Detailed distribution of immunohistochemical staining score along the entire series of 37 ALK FISH-positive cases according to the 3 different clones (ALK1, 5A4, D5F3).

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques: Immunohistochemical staining, Staining, Clone Assay

Crosstabulation considering 2+ and 3+ cases as positive.

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Crosstabulation considering 2+ and 3+ cases as positive.

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques:

Example of invasive lung adenocarcinoma with acinar pattern on surgical specimen (A, haematoxylin-eosin staining) showing weak positivity (score 1+) (B, immunohistochemistry) with clone ALK1, moderate positivity (score 2+) (C, immunohistochemistry) with clone 5A4 and strong positivity (score 3+) (D, immunohistochemistry) with clone D5F3.

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Example of invasive lung adenocarcinoma with acinar pattern on surgical specimen (A, haematoxylin-eosin staining) showing weak positivity (score 1+) (B, immunohistochemistry) with clone ALK1, moderate positivity (score 2+) (C, immunohistochemistry) with clone 5A4 and strong positivity (score 3+) (D, immunohistochemistry) with clone D5F3.

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques: Staining, Immunohistochemistry

Example of metastatic adenocarcinoma on cell-block from pleural effusion (A, haematoxylin-eosin staining) showing weak positivity (score 1+) (B, immunohistochemistry) with clone ALK1, moderate positivity (score 2+) (C, immunohistochemistry) with clone 5A4 and strong positivity (score 3+) (D, immunohistochemistry) with clone D5F3.

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Example of metastatic adenocarcinoma on cell-block from pleural effusion (A, haematoxylin-eosin staining) showing weak positivity (score 1+) (B, immunohistochemistry) with clone ALK1, moderate positivity (score 2+) (C, immunohistochemistry) with clone 5A4 and strong positivity (score 3+) (D, immunohistochemistry) with clone D5F3.

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques: Blocking Assay, Staining, Immunohistochemistry

Crosstabulation considering only 3+ cases as positive.

Journal: Pathologica

Article Title: Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

doi: 10.32074/1591-951X-756

Figure Lengend Snippet: Crosstabulation considering only 3+ cases as positive.

Article Snippet: ALK IHC assay was performed using 3 different clones: Novocastra mouse monoclonal antibody p80 ALK (clone 5A4, Leica Biosystems, Newcastle Upon Tyne, United Kingdom); Companion Diagnostic Kit Ventana anti-ALK rabbit monoclonal primary antibody (clone D5F3, Cell Signaling Technology); mouse monoclonal anti-human CD246 (clone ALK1, Dako/Agilent, Carpentaria, CA).

Techniques:

Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the ALK1/2/3/6 inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 inhibitory antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.

Journal: Molecular Oncology

Article Title: Hepsin promotes breast tumor growth signaling via the TGFβ‐EGFR axis

doi: 10.1002/1878-0261.13545

Figure Lengend Snippet: Hepsin regulates EGFR signaling in a TGFβ‐dependent manner. (A) Western blot comparison of total‐EGFR (tEGFR) levels in control MCF10A pL6‐Ctrl ( N = 3) and MCF10A pL6‐TGFβ1V5, V5‐tagged TGFβ1 overexpressing cells ( N = 3), which secrete around 80–100 pg·mL −1 of TGFβ1. Data are presented as mean ± SD. Significance was tested using the student's t‐test. (B) Western blot analysis of tEGFR in control doxycycline‐ (DOX − ) ( N = 3) and hepsin overexpressing (DOX + ; N = 3) MCF10A‐ pIND20‐HPN pL6‐ TGFβ1V5 cells. Data are presented as mean ± SD. Significance was tested with the student's t ‐test. (C) Western blot analysis of tEGFR, phospho‐SMAD2/3 (pSMAD2/3), and total‐SMAD2 (tSMAD2) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with ALK5 inhibitor (i) (10 μ m RepSox, N = 4) and ALK4/5/7 inhibitor (5 μ m A‐83‐01, N = 3) or DMSO (control, N = 4) for 48 h with (DOX + ; 1 μg·mL −1 ) or without (DOX − , control) hepsin overexpression (the numbers below the tEGFR blot indicate loading normalized values for tEGFR band intensity fold leftmost control lane). The histogram data are presented as mean ± SD. Significance was tested using unpaired t ‐test. (D) Western blot analysis of phospho‐EGFR (pEGFR), tEGFR, total‐MAPK (tMAPK) and phospho‐MAPK (pMAPK), and vinculin (loading control) in MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells. The cells were treated with the ALK1/2/3/6 inhibitor (i) (10 μ m K02288), EGFR inhibitor (i) (10 μ m Erlotinib), and DMSO (control) for 48 h. Data are presented as mean ± SD. Significance was tested with the student's t ‐test ( N = 3). (E) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN cells and MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 (overexpressing TGFβ1) cells grown in 3D culture for 2 weeks with (DOX + ; 1 μg·mL −1 ) or without (DOX − ) induction of hepsin overexpression. Cells were treated with 10 μ m EGFR or ALK1/2/3/6 inhibitor for 2 weeks as indicated in the figure. Scale bar represents 100 μm. (F) Phase‐contrast microscopy images of MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cells grown in 3D culture for 2 weeks in the presence of ALK1 inhibitory antibody (25 μg·mL −1 ). Scale bar represents 50 μm. For E and F, the experiments were repeated three times, with at least 100 epithelial structures counted per group in each repeat (one dot represents one structure), and black lines denote the mean values of each group. Significance was tested using the student's t ‐test. (G) Western blot analysis of the mitotic marker phospho histone H3 (pH3 S10) MCF10A‐pIND20‐HPN pL6‐TGFβ1V5 cell line grown in 3D culture in the presence of the EGFRi (1 μ m , 24 h) or DMSO. The quantification of blots of three independent experiments is shown in the graph (on the right), where the black lines denote the mean of each group. Significance was tested using the student's t ‐test. ns, not significant.

Article Snippet: The inhibitors used in this study were K02288 (Selleckchem, S7359, Houston, TX, USA), Galunisertib/LY2157299 (Selleckchem, S2230), RepSox (Sigma‐Aldrich/Merck, R0158), A‐83‐01 (MedChem Express, HY‐10432, Monmouth Junction, NJ, USA), Erlotinib (EGFRi, Selleckchem, S1023), and human ALK1 inhibitory antibody (R&D Systems, MAB3701).

Techniques: Western Blot, Comparison, Control, Over Expression, Microscopy, Marker